Linked Life Data

7288290

Source:http://linkedlifedata.com/resource/pubmed/id/7288290

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1981-12-22
pubmed:abstractText
Isolated rat livers were perfused at 37 degrees C with blood-free, defined medium containing delipidized bovine serum albumin (BSA), BSA-oleate, or BSA-palmitate. Very low density lipoproteins (VLDL) were isolated from the perfusate at 12 degrees C and lipid components were extracted and purified. Differential scanning calorimetry indicated multiple phase alterations in intact VLDL. The extracted triglycerides exhibited phase alterations at similar temperatures as the intact VLDL. The small quantities of cholesteryl esters present in the VLDL generally did not greatly affect the VLDL triglyceride transitions. The extracted phospholipids showed detectable transitions that were abolished by cholesterol at mole ratios found in the respective VLDL. The phase behavior of VLDL and its component triglycerides was associated with the degree of unsaturation of the infusate fatty acid, and by variation of fatty acid chain length. The structural differences between VLDL lipid fractions noted by DSC were also monitored by fluorescence probes. The data indicated that in the intact VLDL the physical properties of the 'interior core' lipids can affect the properties of the 'surface monolayer'. In addition, Arrhenius plots of corrected fluorescence indicated that trans-parinarate and diphenyl-hexatriene detected different characteristic breakpoint temperatures in the phospholipids. The breakpoints of triglycerides were highly dependent on the type of fatty acid in the infusate, but were similar to those noted in intact VLDL. Finally, the breakpoints in Arrhenius plots of fluorescence probe parameters did not necessarily coincide with onset or end temperatures of DSC transitions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
838-51
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Differential scanning calorimetry and fluorescence probe investigations of very low density lipoprotein from the isolated perfused rat liver.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't