Linked Life Data

7285280

Source:http://linkedlifedata.com/resource/pubmed/id/7285280

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1981-12-15
pubmed:abstractText
Male rats were treated with [ring-3H]N-acetyl-2-aminofluorene and sacrificed at different periods of time after a single i.p. dose. Chromatin was isolated from liver homogenates and treated with RNAse and proteinase K. The resulting crude DNA was purified as the N-hexadecyl-N-trimethylammonium salt. The amounts of 2-aminofluorene and N-acetyl-2-aminofluorene bound to carbon-8 of guanine were determined in the DNA via acid hydrolysis and high pressure liquid chromatography of the hydrolysate. These two major interaction products of the carcinogen decreased rapidly during the first 2 weeks but in the second 2 weeks the decrease of both interactions was much smaller and approximately 15% of the amount that was bound after 24 h remained persistently bound to the DNA. Differences in liver DNA binding after 24 h were observed between male rats of the strain R-Amsterdam (Wistar-related) and Sprague Dawley males. At equal dose levels of N-acetyl-2-aminofluorene, liver DNa of Sprague Dawley rats contained 1/3 of the amount of N-(guanin-8-yl)-2-aminofluorene and 1/2 of the amount of N-(guanin-8-yl)-N-acetyl-2-aminofluorene present in liver DNA of the R-Amsterdam strain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
737-40
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Partial persistency of 2-aminofluorene and N-acetyl-2-aminofluorene in rat liver DNA.
pubmed:publicationType
Journal Article