Linked Life Data

7276115

Source:http://linkedlifedata.com/resource/pubmed/id/7276115

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-11-22
pubmed:abstractText
The systematic separation of medium-sized biologically active peptides by high-performance liquid chromatography (HPLC) is described. Three steps are involved: first, high-performance sodium dodecyl sulphate (SDS) gel chromatography on a newly developed column. TSK-GEL 2000SW; secondly, ion-pair reversed-phase HPLC using stepwise elution mobile phases containing SDS and tetrabutylammonium phosphate; thirdly, high-performance cation-exchange chromatography on a Partisil SCX column for purification, using stepwise gradient elution with volatile buffers. Removal of SDS was possible in the final step. This systematic method is fast, reproducible and gives excellent separations and recoveries.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
213
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-86
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Systematic separation of medium-sized biologically active peptides by high-performance liquid chromatography.
pubmed:publicationType
Journal Article