Linked Life Data

7271362

Source:http://linkedlifedata.com/resource/pubmed/id/7271362

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-10-29
pubmed:abstractText
An optical technique for simultaneous assessment of pH and motility in ram semen was described. Motility was evaluated by the measurement of changes in intensity of reflected light from semen samples illuminated with 366 nm light. pH was measured by using a pH-sensitive fluorescent indicator (umbelliferone). From the changes in fluorescence intensity (450 nm) as a function of the pH of the semen, a calibration curve was constructed. The relationship between pH changes and motility in various metabolic conditions were described. Different kinetics for pH changes occurred when sperm motility was driven by both mitochondrial respiration and fructolysis or by either one of them. An analysis of the fluorescence signal from extracellular or cellular fractions of semen suggested that the pH measurement reflected extracellular pH.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0148-5016
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-33
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
A fluorometric-reflectometric technique for simultaneous measurement of pH and motility in ram semen.
pubmed:publicationType
Journal Article