Linked Life Data

7264549

Source:http://linkedlifedata.com/resource/pubmed/id/7264549

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1981-10-29
pubmed:abstractText
Two-cell and four-cell mouse embryos were cultured in Cytochalasin D (CD) for 40-48 h. They were fixed for light and electron microscopy at various times after washing off the CD. Cleavage-arrested embryos in CD had well separated blastomeres but by 1 h from washing the embryos had compacted, in most cases without undergoing cell division. By 2 h after release from arrest one blastomere of the 2-cell arrested embryos had become crescent shaped and at 4-5 h the crescent-shaped blastomere had started to spread over the surface of the other rounded blastomere. This process continued until by 16-24 h from explantation to fresh medium one blastomere had almost completely engulfed the other. A similar process occurred in 4-cell arrested and released embryos. At this stage the embryos had accumulated fluid and become blastocyst-like vesicles. In 20% of 2-cell and 4-cell embryos one or two blastomeres underwent one cell division after release from arrest. Serial sections of these embryos lead to the conclusion that one or both progeny of the first cell to divide tended to be engulfed by the later dividing or non-dividing cell(s). These results are discussed in relation to the differentiation of ICM and trophectoderm in blastocysts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-0752
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-45
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Morphogenetic analysis of changing cell associations following release of 2-cell and 4-cell mouse embryos from cleavage arrest.
pubmed:publicationType
Journal Article