Linked Life Data

7263297

Source:http://linkedlifedata.com/resource/pubmed/id/7263297

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1981-10-14
pubmed:abstractText
The absorbances of skeletal muscle fibres at 530 nm in control sections of either fresh or boiled tissue, incubated according to Meijer's semipermeable membrane technique for acid phosphatase, increase linearly with incubation time. Moreover, the absorbances continue to increase even after the incubation has been terminated by immersion of the sections in formalin. The comparatively weakly absorbing yellow reaction product originally formed is transformed with 24 h of mounting the sections into a strongly absorbing purple material with an absorption maximum at 570 nm. The original yellow product seems to be hexazotised Pararosanaline adsorbed onto tissue proteins. The formation of the purple product can be prevented by treating control sections immediately after incubation with 70% ethanol for 30 min at room temperature in place of formalin. Ethanol stabilises the yellow non-specific reaction product but does not extract it from tissue sections. The ethanol treatment thus seems useful for improving the validity of Meijer's technique.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5564
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
585-98
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. II. Non-specific reactions in control sections.
pubmed:publicationType
Journal Article