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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1981-10-29
|
| pubmed:abstractText |
NAD-dependent formate dehydrogenase was purified from a cell-free extract of methanol-consuming yeast Candida methylica, using chromatography on DEAE-cellulose and gel-filtration on Sephadex G-200. The enzyme is electrophoretically homogeneous, consists of two identical subunits with molecular weight of 46,000 and is active within the pH range of 6-9; the Km values for NAD and formate are 1 . 10(-4) and 1,3 . 10(-2) M, respectively. Formate dehydrogenase is inhibited by p-chloromercurybenzoate, iodoacetate, dithionitrobenzoate and azide.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0320-9725
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
46
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1119-26
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:7260197-Aldehyde Oxidoreductases,
pubmed-meshheading:7260197-Candida,
pubmed-meshheading:7260197-Formate Dehydrogenases,
pubmed-meshheading:7260197-Hydrogen-Ion Concentration,
pubmed-meshheading:7260197-Kinetics,
pubmed-meshheading:7260197-Molecular Weight,
pubmed-meshheading:7260197-NAD
|
| pubmed:year |
1981
|
| pubmed:articleTitle |
[Isolation and properties of NAD-dependent formate dehydrogenase from the yeast Candida methylica].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|