Linked Life Data

7258736

Source:http://linkedlifedata.com/resource/pubmed/id/7258736

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-9-15
pubmed:abstractText
Serum from a patient who had experienced several episodes of anaphylaxis after eating shrimp was used to monitor the isolation of a major allergen from shrimp, which was then tested with sera from 10 other shrimp-reactive patients. The allergen was isolated from a fresh extract of boiled shrimp by gel filtration and from raw shrimp by gel filtration and agarose electrophoresis. The purity of the allergen was established by crossed immunoelectrophoresis against rabbit and guinea pig antisera; it gave only a single peak in each case. The native allergen was excluded on Sephadex G-200 but showed a single band at 38000d in SDS-PAGE with or without reduction. The isoelectric point was about 4.5. The allergen contained about 4% carbohydrate and only 7 Tyr and 9 Phe out of 341 amino acid residues. Using the RAST with 11 allergic sera, the allergen gave a correlation coefficient of 0.98 with whole cooked shrimp extract and of 0.66 with raw shrimp extract. The allergen was a potent inhibitor of RAST to both cooked and raw shrimp. A second, weaker allergen of 21000d chain size was isolated from raw shrimp in highly purified form.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0003-4738
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17-22
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
The major heat stable allergen of shrimp.
pubmed:publicationType
Journal Article