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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1979-2-23
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pubmed:abstractText |
Previously it was shown that the tryptic digestion of human IgM at 65 C yield Fc'mu fragments in addition to Fabmu and (Fc)5mu fragments. This Fc'mu was found to be derived from the Cmu4 domain of the mu-chain. Additional studies were done on the effects of tryptic digestion time and temperature on the proteolytic process of IgM, IgM digestion by 2% trypsin at 65 C produced (Fc)8mu and Fc'mu in approximately a 3:2 ratio. While an appreciable amount of intact (Fc)5mu was present along with Fc'mu in the IgM digest, no residual "(Fc)5mu fragment" with its C-terminal segment missing was found. If the temperature of digestion is held constant at either 56 C or 60 C and the digestion time is varied from 20 to 90 min, there is also progressive cleavage of IgM with a concomitant increase in the yield of Fc'mu. It appears that the tryptic digestion of IgM is a stepwise process and that the primary cleavage of IgM occurs at Arg-325(2) of all ten mu-chains and the (Fc)5mu is subsequently degraded to Fc'mu fragments by secondary cleavage.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0040-4675
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-93
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:725798-Alkylation,
pubmed-meshheading:725798-Amino Acid Sequence,
pubmed-meshheading:725798-Chromatography, Gel,
pubmed-meshheading:725798-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:725798-Hot Temperature,
pubmed-meshheading:725798-Humans,
pubmed-meshheading:725798-Immunoglobulin Fc Fragments,
pubmed-meshheading:725798-Immunoglobulin M,
pubmed-meshheading:725798-Oxidation-Reduction,
pubmed-meshheading:725798-Time Factors,
pubmed-meshheading:725798-Trypsin
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pubmed:year |
1978
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pubmed:articleTitle |
Primary and secondary tryptic cleavages of human IgM at high temperature.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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