Linked Life Data

7254320

Source:http://linkedlifedata.com/resource/pubmed/id/7254320

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5820
pubmed:dateCreated
1981-9-15
pubmed:databankReference
pubmed:abstractText
Metallothioneins are small cysteine-rich proteins that bind heavy metals such as zinc, cadmium, copper and mercury. Recent interest in these proteins has focused on the part they play in zinc metabolism and heavy metal detoxification. Our interest in metallothionein genes stems largely from the observations that these proteins are inducible by both heavy metals and glucocorticoid hormones. To explore the regulation of these genes, we have isolated cDNA and genomic clones corresponding to mouse metallothionein-I (MT-I), and have used them to show that both inducers act at the transcriptional level in vivo and in a wide variety of cell lines. We have also shown that the MT-I gene is amplified during selection for cadmium resistance. To investigate the mechanisms of gene regulation, knowledge of the primary DNA sequence is necessary. Here we present the entire sequence of mouse MT-I gene along with approximately 300 bases of 5' flanking region that presumably includes promoter and regulatory sites. The 5' mRNA sequence, defined by S1 nuclease mapping, was combined with sequences of the coding and 3' untranslated regions obtained previously to allow a computer prediction of the most stable secondary structure of MT-I mRNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
292
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
267-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Structure of mouse metallothionein-I gene and its mRNA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't