Linked Life Data

7254219

Source:http://linkedlifedata.com/resource/pubmed/id/7254219

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-9-15
pubmed:abstractText
In mutagenicity studies a rapid detection of chromosomal damage in mammalian germ cells would be very valuable. Encouraged by the usefulness of the bone-marrow micronucleus test, we applied analogous method to the assay of micronuclei induced during meiotic reduction divisions in the adult male rat by X-irradiation. The micronuclei were observed in early post-meiotic cells which were enriched using a transillumination phas-contrast microscopic method. The frequency of micronuclei was scored at various dose levels and at various time intervals. The results indicate a linear increase in the frequency of micronuclei 24 h after X-irradiation with doses of 0, 10, 50, 150, 300 and 600 rad. The highest frequency of micronuclei was observed after 900 rad whereas lower frequencies were found after 1200 rad. The lowest dose giving a statistically significant increase above the control level was 50 rad. The stages of meiosis showed different sensitivities to the chromosome-breaking action of X-rays. The maximal incidence of micronuclei was found 18 h after irradiation which was considered to reflect the great radiosensitivity of diakinesis-metaphase I. The anesthetized group of control animals showed a slightly higher frequency of micronuclei than the non-anesthetized controls. Potentials of the new method for mutagen testing are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Meiotic micronuclei induced by X-rays in early spermatids of the rat.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't