Linked Life Data

7252424

Source:http://linkedlifedata.com/resource/pubmed/id/7252424

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1981-9-25
pubmed:abstractText
The promastigote form of Leishmania donovani and Leishmania tropica, the etiologic agents of visceral and cutaneous leishmaniasis, respectively, readily parasitize unstimulated J774G8 macrophage-like cells, whereas 80-95% of the same promastigotes are killed within resident macrophages from normal BALB/c mice. This striking difference in intracellular anti-leishmanial activity correlated closely with the capacity to generate toxic oxygen intermediates. Thus, after triggering with phorbol myristate acetate or phagocytosis of zymosan or promastigotes, 90% of the J774G8 cells failed to reduce nitroblue tetrazolium, and released 5-10-fold less O2- and H2O2 than BALB/c macrophages. Exposure to concanavalin A-stimulated lymphokine, however, effectively enhanced the oxidative response of J774G8 cells, and, similarly, induced intracellular anti-leishmanial activity. Inhibiting macrophage H2O2 production consistently decreased the killing of Leishmania by lymphokine-treated J774G8 cells. These observations illustrate the usefulness of examining homogeneous macrophage cell lines that are deficient in a particular effector function, and also serve reemphasize the important role of oxygen intermediates in the microbicidal response of mononuclear phagocytes to intracellular parasites.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-1089721, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-209122, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-210935, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-216763, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-216766, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-286323, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-376774, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-512587, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-6252274, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-6256463, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-7252418, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-7403880, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252424-949553
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1690-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Interaction of Leishmania with a macrophage cell line. Correlation between intracellular killing and the generation of oxygen intermediates.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't