Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-9-15
pubmed:abstractText
A fragment of activated Hageman factor (HFf) has been demonstrated to activate the classical pathway of complement in a manner that is analogous to complement activation by antigen-antibody complexes or aggregated IgG. Thus C1, C4, C2, C3, and C5 were found to be depleted on addition of HFf to serum. The reduction of serum hemolytic activity was maximal upon addition of 5 micrograms HFf and an incubation time of 60 min at 37 degrees C. Consumption of the total complement activity and of the individual components proceeded in a dose-dependent fashion. No comparable activity was observed when equimolar concentrations of either the native Hageman factor (HF) or two-chain activated form of Hageman factor (HFa) were incubated with serum. Further, the ability of HFf to convert serum C3 and C4 was similar to that of aggregated IgG as assessed by immunoelectrophoresis. This function of HFf appeared to be independent of plasminogen (or plasmin) since plasminogen-free serum was indistinguishable from normal serum. Radial double immunodiffusion experiments using antiserum to C1q, C1r, and C1s on HFf-treated serum demonstrated the dissociation of the C1 trimolecular complex, with concomitant reduction of C1r antigenicity that is indicative of C1 activation. Thus, HFf appears to lead to C1 activation upon incubation with serum or when incubated with partially purified C1. This may represent a control link between activation of the intrinsic coagulation-kinin pathway and the initiation of the classical complement cascade.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-1201121, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-123251, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-14078003, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-14106311, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-14316946, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-362477, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-4159289, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-4178758, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-4225264, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-4261121, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-4349137, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-438335, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-4547783, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-4663514, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-4997106, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-5299945, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-5435787, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-5470521, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-5475635, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-5477337, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-5508377, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-5926456, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-5960883, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-6019133, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-6158260, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-632748, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-6898641, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-7391081, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-75238, http://linkedlifedata.com/resource/pubmed/commentcorrection/7252410-823964
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
665-76
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Activation of the classical pathway of complement by Hageman factor fragment.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.