Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1981-9-15
|
pubmed:abstractText |
The cytoplasmic NADPH-dependent prostaglandin D2 11-keto reductase from rabbit liver was purified by a series of chromatographic procedures including isoelectric focusing. The enzyme catalyzed the conversion of prostaglandin D2 to prostaglandin F2 alpha and had a pH optimum of 7.0-7.5, and an isoelectric point of 5.8. The molecular weight was estimated to be 66 000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Enzymic activity was time and concentration dependent and required NADPH as cofactor.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0006-3002
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
14
|
pubmed:volume |
659
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
169-78
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:7248317-Animals,
pubmed-meshheading:7248317-Chromatography, DEAE-Cellulose,
pubmed-meshheading:7248317-Hydroxyprostaglandin Dehydrogenases,
pubmed-meshheading:7248317-Isoelectric Focusing,
pubmed-meshheading:7248317-Liver,
pubmed-meshheading:7248317-Male,
pubmed-meshheading:7248317-NADP,
pubmed-meshheading:7248317-Platelet Aggregation,
pubmed-meshheading:7248317-Prostaglandins D,
pubmed-meshheading:7248317-Prostaglandins F,
pubmed-meshheading:7248317-Rabbits
|
pubmed:year |
1981
|
pubmed:articleTitle |
Purification and partial characterization of prostaglandin D2 11-keto reductase in rabbit liver.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|