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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1981-8-10
|
| pubmed:abstractText |
Rat intermediate pituitary cells maintained in culture synthesize the same forms of beta-endorphin observed in intermediate pituitary extracts. Biosynthetically labeled intermediate pituitary beta-endorphin-sized material was fractionated by ion exchange chromatography on sulfopropyl-Sephadex and the identities of the major peaks were determined by co-chromatography with synthetic marker peptides, gel filtration, and analysis of pronase, chymotrypsin, and trypsin digests. Peaks of alpha-N-acetyl-beta-endorphin(1-27), alpha-N-acetyl-beta-endorphin(1-31), and beta-endorphin(1-31) were identified and a fourth peak (eluting from the sulfopropyl-Sephadex column at 0.18 M NaCl) was tentatively identified as alpha-N-acetyl-beta-endorphin(1-26). Analysis of beta-endorphin synthesized in the presence of [35S]methionine and [3H]histidine confirmed the absence of His in the material eluting at 0.18 M NaCl. Based on both steady labeling and pulse-chase incubations, beta-endorphin(1-31) was the first form of labeled beta-endorphin-sized material to appear in cell extracts. This molecule was quickly N-acetylated on its NH2-terminal tyrosine residue and was then more slowly converted to alpha-N-acetyl-beta-endorphin(1-27) and then to alpha-N-acetyl-beta-endorphin(1-26).
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
256
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5689-95
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading | |
| pubmed:year |
1981
|
| pubmed:articleTitle |
Further analysis of post-translational processing of beta-endorphin in rat intermediate pituitary.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|