Linked Life Data

7237289

Source:http://linkedlifedata.com/resource/pubmed/id/7237289

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1981-8-20
pubmed:abstractText
The K+, Na+, and Mg2+ contents of Methanospirillum hungatei and of the thermophile Methanobacterium thermoautotrophicum were determined at various phases of growth. The intracellular K+ content of exponential phase cells of M. thermoautotrophicum (approximately 780 mM) was 5.4-fold higher than in M. hungatei, and decreased gradually as the culture entered the stationary phase. Both methanogens concentrated Mg2+, exhibiting an increased content as the cultures aged. Comparisons among extraction methods showed that most of the internal K+ was readily released, but a minimum of half of the Mg2+ in M. hungatei, and most of the M2+ in M. thermoautotrophicum, was in a bound form. Exponential phase of cells of M. hungatei established an intracellular level of Na+ lower than the outside medium, but the thermophile concentrated Na+. Dextran, inulin, sucrose, and glucose penetrated cell pellets to varying degrees and could be used to measure the space corresponding to cytoplasm and to cell wall permeability barriers. L-Phenylalanine penetrated fully and acetate accumulated in both methanogens. Acetate uptake in cell suspensions of M. hungatei was fully inhibited by oxygen. N-ethylmaleimide, or N,N'-dicyclohexylcarbodiimide, but was not affected by the proton conductor carbonylcyanide p-trifluoromethoxyphenyl-hydrazone. L-Malate, which penetrated M. hungatei cells poorly, was metabolized to glutamate, indicating the presence of an incomplete reductive carboxylic acid cycle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0008-4166
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
444-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
K+, Na+, and Mg2+ content and permeability of Methanospirillum hungatei and Methanobacterium thermoautotrophicum.
pubmed:publicationType
Journal Article, Comparative Study