Linked Life Data

7237259

Source:http://linkedlifedata.com/resource/pubmed/id/7237259

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1981-8-20
pubmed:abstractText
Chloride liquid ion-exchange microelectrodes were used to measure intracellular chloride activity (aCl)i or rabbit papillary muscle in vitro. In HCO3- -- CO2 buffered Ringer (aCl)i was 15.2 +/- 0.6 mM (n = 40) at pH 7.3 and 15.4 +/- 0.3 mM (n = 29) at pH 6.8. In HEPES-buffered Ringer (aCl)i was 24.1 +/- 0.6 mM (n = 21) at pH 7.3 and 20.0 +/- 0.3 mM (n = 17) at pH 6.9. If Cl was distributed passively across the membrane (aCl)i should be about 3 mM. From total muscle Cl analysis and [14C]sorbitol extracellular space measurements we calculate an apparent intracellular Cl concentration of 71 mM. As this estimate is three times larger than the measured free Cl in the cytoplasm we conclude that a significant fraction of muscle Cl must be bound or compartmentalized. Analysis of 36Cl efflux from the papillary muscle indicates the presence of two compartments. The compartment with the slower efflux rate (2.0 X 10(-2) min-1) contains about 25% of the intracellular Cl, which compares with the estimation (21%) of the free intracellular CL fraction obtained from the Cl microelectrode measurements. Our data permit us to conclude that about 37% of the muscle Cl is bound and that it is probably located extracellularly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0008-4212
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
479-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Intracellular Cl activity, Cl binding, and 36Cl efflux in rabbit papillary muscle.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't