Linked Life Data

7236613

Source:http://linkedlifedata.com/resource/pubmed/id/7236613

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1981-8-27
pubmed:abstractText
Tb3+ and Eu3+ bound to troponin C were detected by (1) changes in the fluorescence of the tyrosine chromophore of the protein or (2) the luminescence of the ions themselves excited by energy transfer from the protein or by direct excitation using a pulsed laser light source [Horrocks, W. DeW., Jr., & Sudnick, D. R. (1979) Science (Washington, D.C.) 206, 1194-1196]. Titrations carried out in the absence and presence of Ca2+ suggest two classes of binding sites (two sites in each class) for the lanthanides, corresponding to the high- and the low-affinity sites for Ca2+. Computer analysis, assuming competition between Ca2+ and the lanthanide, using the binding constants of Ca2+ for TnC [Potter, J. D., & Gergely, J. (1975( J. Biol. Chem. 250, 4628-4633] yields 5.2 X 10(8) M-1 and 9.7 X 10(6) M-1 for Tb3+ and 4.7 X 10(9) M-1 and 5.3 X 10(7) M-1 for Eu3+, for the high- and the low-affinity sites, respectively. From lifetimes of laser-induced luminescence in H2O and in D2O, the number of water molecules coordinated to Eu3+ was two at the high and three at the low affinity sites.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2439-44
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Binding of lanthanide ions to troponin C.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't