Linked Life Data

7230838

Source:http://linkedlifedata.com/resource/pubmed/id/7230838

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-7-20
pubmed:abstractText
The presence of tele-methylhistamine (t-MH), the metabolite of histamine in rat brain, and its relationship to putative histaminergic transmission have been the subjects of recent work. We modified the GCMS method of Hough et al. (1979) to enhance both sensitivity and reproducibility. The substitution of KOH for NaOH to extract t-MH considerably improved the recovery. Evaporation of the extract in 0.2 N HCl, as used in the earlier method, reduced the formation of heptafluorobutyryl derivative; substitution with 0.01 N HCl more than doubled the recovery of the derivative. The derivatization procedure itself was changed, the new method exhibiting significantly improved reproducibility. Standard curve of t-MH obtained at different times after derivatization were indistinguishable. The modified method is capable of measuring less than 1 pmole of t-MH. The t-MH content found in nine rat brain regions agree with previously reported values.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0160-5402
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
143-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
An improved GCMS method to measure tele-methylhistamine.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.