Linked Life Data

7229386

Source:http://linkedlifedata.com/resource/pubmed/id/7229386

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-7-23
pubmed:abstractText
The rapid separation of large numbers of viable thymus cells from AKR mice bearing transplanted or spontaneous thymic lymphomas was achieved by centrifugal elutriation. Separation of more than 3 x 10(8) cells from either a transplanted lymphoma, designated 720, or from a spontaneous thymic lymphoma, required only 15 min. Unfractionated thymus cells obtained from mice bearing the transplanted lymphoma consisted of 80% lymphoma cells (by immunofluorescence for the viral protein, gp70) and 20% normal cells. Fractions of slowly sedimenting cells consisted almost exclusively of normal cells (95%) with modal volumes of 95 micrometers cubed. Fractions of rapidly sedimenting cells consisted of 95% tumor cells with volumes of 150-400 micrometers cubed. The slowly sedimenting cells were almost exclusively (98%) in the GO- or G1-phase. Fractions of rapidly sedimenting cells contained up to 55% S-phase and up to 30% G2-phase cells. Intermediate fractions contained mixtures of normal cells and small GO- or G1-phase tumor cells. Thymidine uptake by the separated cells was determined. The fractions containing normal cells showed little thymidine uptake after 4 and 48 h in culture, while the fractions of tumor cells showed high levels of incorporation. In contrast to the high levels of thymidine uptake by the tumor cell fractions after 48 h in culture, there was little uptake by the unseparated cell suspension, suggesting a possible interaction between normal and tumor cells during the culture period.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-301
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Separation of AKR mouse thymus lymphoma from normal thymic cells by centrifugal elutriation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't