| pubmed-article:7217336 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C0237868 | lld:lifeskim |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C0020852 | lld:lifeskim |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C0020861 | lld:lifeskim |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C0035448 | lld:lifeskim |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C0036679 | lld:lifeskim |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C2346501 | lld:lifeskim |
| pubmed-article:7217336 | lifeskim:mentions | umls-concept:C0332296 | lld:lifeskim |
| pubmed-article:7217336 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7217336 | pubmed:dateCreated | 1981-6-23 | lld:pubmed |
| pubmed-article:7217336 | pubmed:abstractText | The proposed method was designed to replace the tedious and difficult separation of immunoglobulin M (IgM) from IgG by sucrose gradient sedimentation. In this method, a 250-microliter portion of serum diluted 20-fold was passed through a small column of quaternary aminoethyl-Sephadex A-50 ion exchanger. IgG was not retained, but additional washes were required to remove all but 5%. A second buffer-eluting fluid recovered an average of 80% of the original IgM in a defined dilution. The entire operation took 15 min. The efficiency of this process was evaluated by the following: (i) radial immunodiffusion measurements of IgG and IgM; (ii) recovery studies of isohemagglutinins; and (iii) demonstrated removal of interference by the rheumatoid factor. The method was applied successfully to distinguish rubella IgM antibody. | lld:pubmed |
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| pubmed-article:7217336 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7217336 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7217336 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7217336 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7217336 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:7217336 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:7217336 | pubmed:author | pubmed-author:JohnsonR... | lld:pubmed |
| pubmed-article:7217336 | pubmed:author | pubmed-author:LibbyRR | lld:pubmed |
| pubmed-article:7217336 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7217336 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:7217336 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7217336 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7217336 | pubmed:pagination | 451-4 | lld:pubmed |
| pubmed-article:7217336 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:7217336 | pubmed:meshHeading | pubmed-meshheading:7217336-... | lld:pubmed |
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| pubmed-article:7217336 | pubmed:meshHeading | pubmed-meshheading:7217336-... | lld:pubmed |
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| pubmed-article:7217336 | pubmed:meshHeading | pubmed-meshheading:7217336-... | lld:pubmed |
| pubmed-article:7217336 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:7217336 | pubmed:articleTitle | Separation of immunoglobulin M (IgM) essentially free of IgG from serum for use in systems requiring assay of IgM-type antibodies without interference from rheumatoid factor. | lld:pubmed |
| pubmed-article:7217336 | pubmed:publicationType | Journal Article | lld:pubmed |
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