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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1981-6-23
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pubmed:abstractText |
The proposed method was designed to replace the tedious and difficult separation of immunoglobulin M (IgM) from IgG by sucrose gradient sedimentation. In this method, a 250-microliter portion of serum diluted 20-fold was passed through a small column of quaternary aminoethyl-Sephadex A-50 ion exchanger. IgG was not retained, but additional washes were required to remove all but 5%. A second buffer-eluting fluid recovered an average of 80% of the original IgM in a defined dilution. The entire operation took 15 min. The efficiency of this process was evaluated by the following: (i) radial immunodiffusion measurements of IgG and IgM; (ii) recovery studies of isohemagglutinins; and (iii) demonstrated removal of interference by the rheumatoid factor. The method was applied successfully to distinguish rubella IgM antibody.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-14253527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-360776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-368269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-371131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-392050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-4158021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-4202959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-4411735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-4628160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-5094687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-6067560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-670949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-7229043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-7356569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-77280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7217336-811545
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
451-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1980
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pubmed:articleTitle |
Separation of immunoglobulin M (IgM) essentially free of IgG from serum for use in systems requiring assay of IgM-type antibodies without interference from rheumatoid factor.
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pubmed:publicationType |
Journal Article
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