Linked Life Data

7217083

Source:http://linkedlifedata.com/resource/pubmed/id/7217083

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1981-6-25
pubmed:abstractText
Two fluorescent cholesteryl esters, cholesteryl cis-parinarate and cholestatrienyl oleate, have been incorporated into human serum low density lipoprotein (LDL) by two alternative procedures. The core location of the incorporated cholesterol esters and the surface location of cis-parinaric acid and cholestatrienol are verified by fluorescence energy transfer. In these LDL preparations, the incorporated cholesteryl esters exhibit a temperature-dependent induced circular dichroic spectrum at the absorption wavelengths of the chromophore with a temperature range which is similar to the calorimetrically determined thermal transition of LDL. We also examined the induced circular dichroism which arises when cholestatrienyl oleate is dissolved in pure cholesteryl oleate and observed a temperature dependence of the sign and magnitude of the circular dichroic spectrum which correlated with the temperature range of the calorimetric phase transitions. On the basis of these studies, we conclude that below the calorimetric transition, the cholesteryl esters of low density lipoprotein have an organization which is intermediate between isotropic and cholesteric, while above the transition, the organization is more nearly isotropic. Residual environmental chirality experienced by cholesteryl ester acyl chains (cholesteryl parinarate) in the particle core and free sterol (cholestatrienol) in the particle surface could represent specific lipid-protein interactions. These results are discussed in terms of the organization of low density lipoprotein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
256
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4286-92
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Induced circular dichroism of incorporated fluorescent cholesteryl esters and polar lipids as a probe of human serum low density lipoprotein structure and melting.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't