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rdf:type |
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lifeskim:mentions |
umls-concept:C0008151,
umls-concept:C0010572,
umls-concept:C0022827,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0085862,
umls-concept:C0439536,
umls-concept:C0598783,
umls-concept:C1299583,
umls-concept:C1524063,
umls-concept:C1549571,
umls-concept:C1608386,
umls-concept:C2603343
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pubmed:issue |
2
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pubmed:dateCreated |
1981-6-13
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pubmed:abstractText |
A system for measuring chlamydial lipid synthesis was developed with mouse L cells grown in serum-free modified Waymouth 752/l medium in a shaker culture. Host lipid synthesis was reduced approximately 90% when cells were incubated for 24 h in medium containing cycloheximide (2 micrograms/ml). Lipid metabolism was monitored by measuring the incorporation of [3H]isoleucine into the total lipid of normal and infected cells. The results suggested that lipid synthesis of Chlamydia trachomatis lymphogranuloma venereum (LGV-404L) was not inhibited by cycloheximide treatment when the chlamydiae were grown in L cells, whereas host lipid synthesis was inhibited. Chlamydial lipid metabolism began about 6 to 12 h after infection when the noninfectious reticulate body was found and continually increased until the beginning of the appearance of intracellular infectious elementary bodies at 24 to 30 h.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-1095493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-1250851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-13262140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-13671378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-13868224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-14208575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-14449901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-15436424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-329832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-4567659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-4624816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-4856239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-4987309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-5354949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-5449698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-562356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-5640375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7216466-5773017
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
668-73
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1981
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pubmed:articleTitle |
Use of cycloheximide to study independent lipid metabolism of Chlamydia trachomatis cultivated in mouse L cells grown in serum-free medium.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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