Linked Life Data

7200800

Source:http://linkedlifedata.com/resource/pubmed/id/7200800

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1982-8-7
pubmed:abstractText
The association of membrane and cytoplasmic proteins with the cytoskeleton of resting and activated platelets was studied. Glycoproteins were identified by labeling with 125I-labeled lectins (concanavalin A, wheat germ agglutinin, and Lens culinaris). Polypeptides, which are embedded in the lipid bilayer, have been identified by their photolabeling with the lipid-soluble reagent 5-[125I]iodonaphthyl 1-azide (125INA). Cytoplasmic proteins were identified by their photolabeling with the intracellular probe azidofluorescein diacetate. Results indicate that the Triton X-100 residue contains the membrane-associated glycoprotein Ia, a 95 000-dalton protein, and two other acidic proteins of molecular weights of 35 000-40 000, one labeled with 125INA and the other with azidofluorescein diacetate. The presence of part of these proteins in the Triton residue is dependent upon the mode of platelet activation. Glycoproteins IIb and III are embedded in the membrane lipid bilayer but sedimented with the Triton residue only after thrombin activation. Another protein with Mr 70 000, which is highly labeled by 125INA in resting platelets, is found only in the Triton-soluble fraction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1713-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Association of membrane and cytoplasmic proteins with the cytoskeleton in blood platelets.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't