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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1979-2-23
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pubmed:abstractText |
Cold acid phenol extractions (Kirby, K.S. (1968) in Methods in Enzymology, Vol. XII B, pp. 87--99, Academic Press, New York) of homogenates from oocytes, zygotes and larval stages of Ascaris lumbricoides yield predominantly glycogen, some glycogen . phosphate complexes and guanine nucleotides, but only small amounts of RNA. Oocytes contain 10.0 pg of extractable 19 S and 26 S rRNA and 1.1 pg 4 S and 5 S RNA per cell. During the maturation of the oocyte to the zygote stage, the amount of 19 S and 26 S rRNA increases by 57%, and that of 4 S and 5 S RNA by 130%. Larval stages contain 62% more 19 S and 26 S rRNA, and 47% more 4 S and 5 S RNA than zygotes. The amount of the extractable glycogen decreases by about 80% and nucleotides by about 70% from oogenesis to the larval stage. The Kirby extract from homogenates of spermatids contains only traces of glycogen, glycogen . phosphate complexes and nucleotides, and 115 times less RNA per spermatid than oocytes. The nucleotide pool sizes of oocytes, zygotes and larval stages were determined; the pools consist of 71--88% guanine nucleotides. Spermatids contain only adenine nucleotides and an unidentified, nucleotide-like compound.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
521
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
251-66
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:718930-Animals,
pubmed-meshheading:718930-Ascaris,
pubmed-meshheading:718930-Female,
pubmed-meshheading:718930-Glycogen,
pubmed-meshheading:718930-Larva,
pubmed-meshheading:718930-Male,
pubmed-meshheading:718930-Metamorphosis, Biological,
pubmed-meshheading:718930-Oocytes,
pubmed-meshheading:718930-Ovary,
pubmed-meshheading:718930-RNA,
pubmed-meshheading:718930-Ribonucleotides,
pubmed-meshheading:718930-Spermatids
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pubmed:year |
1978
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pubmed:articleTitle |
Quantitative analysis of RNA, glycogen and nucleotides from different developmental stages of Ascaris lumbricoides (var. suum).
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pubmed:publicationType |
Journal Article
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