Linked Life Data

7174693

Source:http://linkedlifedata.com/resource/pubmed/id/7174693

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1983-2-14
pubmed:abstractText
Apolipoprotein B obtained from each of the major density classes of human plasma lipoproteins was investigated immunochemically. Eleven different mouse hybridoma cell lines were generated and selected on the basis of their ability to secrete antibodies that were bound by either very low density or intermediate density lipoproteins. Two of these antibodies appeared to be specific for complex epitopes on apoprotein B, since they were bound by all apoprotein B-containing lipoproteins, but not by apoprotein B after it had been delipidated and electrophoresed on sodium dodecyl sulfate-polyacrylamide gels. The remaining nine hybridoma antibodies were capable of binding to apoprotein B; and appeared to be specific for relatively simple epitopes that were expressed by denatured apoprotein B. The ability of these nine hybridoma antibodies to bind to the denatured apoprotein B species of plasma chylomicrons, very low density lipoproteins, and low density lipoproteins was compared. The apoprotein B species were separated on the basis of their relative mobility in low percentage sodium dodecyl sulfate-polyacrylamide gels and transferred to nitrocellulose paper for reaction with antibody. These binding studies suggest that plasma apoprotein B represents a complex family of immunochemically heterogeneous but related apoprotein species. A schematic summary of the apoprotein B species distribution of epitopes identified by these hybridoma antibodies is presented.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
257
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15213-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Immunochemical heterogeneity of human plasma apolipoprotein B. I. Apolipoprotein B binding of mouse hybridoma antibodies.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't