Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1983-2-14
|
| pubmed:abstractText |
The non-cellular investments were damaged in only 2.8% of rabbit morulae frozen/ thawed in plastic straws. Similar results (4.8%) were obtained with glass ampoules only when morulae were slowly frozen and slowly thawed in siliconized ampoules. Freezing/thawing by other methods and the use of untreated ampoules resulted in high incidence of embryos with damaged investments (41-61%). Morulae frozen/thawed by a method allowing an almost complete elimination of investment injury displayed high survival when slowly frozen and rapidly thawed (90.7%) while slow freezing and slow thawing decreased survival (74.5%). Morulae subjected to rapid freezing were for the first time successfully stored at--196 degrees C. After rapid thawing 59.6% morulae survived. The mechanism of damage to the non-cellular investments of rabbit embryos is discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0015-5500
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
28
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
350-8
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7173451-Animals,
pubmed-meshheading:7173451-Cell Survival,
pubmed-meshheading:7173451-Cleavage Stage, Ovum,
pubmed-meshheading:7173451-Female,
pubmed-meshheading:7173451-Freezing,
pubmed-meshheading:7173451-Morula,
pubmed-meshheading:7173451-Rabbits,
pubmed-meshheading:7173451-Superovulation,
pubmed-meshheading:7173451-Time Factors,
pubmed-meshheading:7173451-Tissue Preservation
|
| pubmed:year |
1982
|
| pubmed:articleTitle |
Protection of non-cellular investments of rabbit morulae stored at--196 degrees C.
|
| pubmed:publicationType |
Journal Article
|