Linked Life Data

7173218

Source:http://linkedlifedata.com/resource/pubmed/id/7173218

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-2-25
pubmed:abstractText
Mitotic PtK1 cells were arrested at nuclear envelope breakdown with the rapidly reversible microtubule inhibitor nocodazole. Addition of 1 microgram/ml nocodazole for 20 min resulted in complete blockage of spindle microtubule assembly but permitted other mitotic events to proceed such as chromosome condensation and cell rounding, typical of a c-mitosis. Upon release from nocodazole, mitosis and cytokinesis were completed approximately 35% faster than in a normal mitosis. The duration of each mitotic stage was compared in untreated and treated and released cells. Metaphase and cytokinesis were dramatically shortened while anaphase was unaffected. Cells treated for shorter periods of time with nocodazole and then released also reduced the length of time to complete mitosis and cytokinesis. The reduction in duration of these events was equivalent to the period of interruption of the mitotic cycle by nocodazole. With respect to the cell division cycle, PtK1 cells display a timing mechanism which is independent of the presence of microtubules.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0171-9335
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
190-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Rapid completion of mitosis and cytokinesis in PtK cells following release from nocodazole arrest.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't