Linked Life Data

7173194

Source:http://linkedlifedata.com/resource/pubmed/id/7173194

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-2-14
pubmed:abstractText
The inactivation of glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus and sturgeon by 3-chloroacetylpyridine--adenine dinucleotide, which is not active as a coenzyme, obeys pseudo-first-order kinetics. The mechanism of inactivation corresponds to an affinity label. Four moles of inactivator are incorporated per mole of tetramer for both enzymes. All the kinetic results strongly support the idea that neither enzyme exhibits negative cooperativity for the non-covalent binding of 3-chloroacetylpyridine--adenine dinucleotide. NAD+ strongly protects both glyceraldehyde-3-phosphate dehydrogenases from inactivation. In the presence of NAD+, the inactivation process seems also to exhibit pseudo-first-order kinetics. The fact that each kinetic experiment can be described by only one pseudo-first-order rate constant is unexpected. The results of thiol titration are in favor of the modification of the essential Cys-149, although another thiol group per monomer, probably Cys-153, becomes titratable on both native alkylated enzymes. This latter result suggests that the alkylation of the Cys-149 by the 3-chloroacetylpyridine--adenine dinucleotide should affect the tertiary structure of these enzymes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:volume
127
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
519-24
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Affinity labeling of glyceraldehyde-3-phosphate dehydrogenase from sturgeon and Bacillus stearothermophilus by 3-chloroacetylpyridine--adenine dinucleotide. Kinetic studies.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't