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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1983-6-23
|
| pubmed:abstractText |
Experiments performed at 25 degrees C, pH 7.4, confirmed that the human red blood cell possesses both Na-independent and Na-dependent transport systems for neutral amino acids. Further evidence for the existence of a major Na-independent pathway for the large neutral amino acids (L-leucine, L-isoleucine, L-phenylalanine, L-valine and L-methionine), designated the 'L-system', was provided from trans-acceleration experiments of L-leucine efflux. Transport via the L-system with respect to kinetics of substrates and stereospecificity was studied. Experiments on inhibition of transport and on kinetics of Na-dependence of uptake of L-alanine and glycine were consistent with Na-dependent amino acid transport being mediated by two different systems, i.e. an ASC-system for L-alanine, L-serine and L-cysteine and a Gly-system for glycine transport. When compared with a 'high capacity/low affinity' pattern of kinetics of the L-system, Na-dependent uptake of L-alanine and glycine was found to exhibit 'low capacity/high affinity' kinetics. The Na-dependence of L-alanine uptake conformed to first order interaction, that of glycine uptake to second order. An effect predominantly on the maximum transport capacity (V12) of the saturable Na-dependent uptake route of L-alanine and glycine, respectively, by a 50% reduction of the extracellular Na+ concentration, was suggested.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0001-6772
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
116
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
321-30
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading | |
| pubmed:year |
1982
|
| pubmed:articleTitle |
Na-independent and Na-dependent transport of neutral amino acids in the human red blood cell.
|
| pubmed:publicationType |
Journal Article
|