Linked Life Data

7163632

Source:http://linkedlifedata.com/resource/pubmed/id/7163632

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-4-7
pubmed:abstractText
Addition of KCN or H2O2 to normal human urine resulted in chemiluminescence (CL). The CL increased with increasing concentrations of KCN and H2O2 and with increasing pH of the reaction mixture. KCN-induced UCL was markedly inhibited by exogenously added ascorbic acid (AA) and to varying degrees by glucose, albumin, tryptophan, hemoglobin and histidine. In contrast H2O2-induced UCL was significantly inhibited only by tryptophan. Mannitol and ethanol (hydroxyl radical scavengers) had little effect on either KCN-or H2O2-induced UCL. The KCN-sensitive and the H2O2-sensitive components were eluted in different positions from a Sephadex G15 column indicating chemically distinct compounds with molecular weights of less than 1500 daltons. We conclude that the UCL produced by H2O2 and KCN arise via interactions with chemically distinct compounds present in the urine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0034-5164
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
339-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
KCN-induced urinary chemiluminescence (UCL): inhibition by ascorbic acid.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't