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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1983-4-7
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pubmed:abstractText |
RNA product distribution obtained during the transcription of poly[d(A-T)] by wheat germ RNA polymerase IIA under various experimental conditions was analyzed by high resolution polyacrylamide gel electrophoresis. Poly[r(A-U)] synthesis proceeded as if wheat germ RNA polymerase II was a non-processive enzyme: a ladder of RNA products of increasing lengths was obtained, which apparently, terminated at every other nucleotide. RNA release was not dependent upon nucleoside triphosphate substrate concentrations. A likely explanation would be that ternary complexes enzyme: DNA: RNA were very much unstable; moreover, oligonucleotides released were not re-used for further elongation by the enzyme.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0014-5793
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
27
|
pubmed:volume |
150
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
477-81
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7160487-DNA-Directed RNA Polymerases,
pubmed-meshheading:7160487-Kinetics,
pubmed-meshheading:7160487-Plants,
pubmed-meshheading:7160487-Poly dA-dT,
pubmed-meshheading:7160487-Polydeoxyribonucleotides,
pubmed-meshheading:7160487-RNA Polymerase II,
pubmed-meshheading:7160487-Transcription, Genetic,
pubmed-meshheading:7160487-Triticum
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pubmed:year |
1982
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pubmed:articleTitle |
Non-processive transcription of poly[d(A-T)] by wheat germ RNA polymerase II.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|