Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-3-17
pubmed:abstractText
The principal zinc binding component in human prostatic fluid and seminal plasma has been found to bind zinc reversible and to be of low molecular weight. Treatment of this zinc containing complex (LMW-Zn) with trypsin and pronase or acid hydrolysis failed to alter its zinc binding properties or gel chromatographic characteristics. Removal of citrate with a combination of citrate lyase, malate dehydrogenase and lactate dehydrogenase did, however, completely abolish its ability to bind zinc. Spectrographical scanning of LMW-Zn preparations obtained by gel chromatography of prostatic fluid or seminal plasma on a Sephadex G-25 column gave identical absorption curves with those obtained by scanning of a zinc-citrate solution. Application of the same analysis to LMW-Zn of human breast milk yielded similar results. It is concluded that citrate is the major low molecular weight zinc ligand in prostatic fluid, seminal plasma and breast milk.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0001-6772
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
67-73
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Zinc and zinc ligands in human seminal plasma. III. The principal low molecular weight zinc ligand in prostatic secretion and seminal plasma.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't