Linked Life Data

7154117

Source:http://linkedlifedata.com/resource/pubmed/id/7154117

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1983-3-24
pubmed:abstractText
Seven day chick embryo cerebral cortical neurons cultured at low density (10,000 cells/16 mm well) in serum-free, defined medium in polylysine-coated wells fail to extend neurites and assume a flattened phase-dark morphology. Addition of serum-free, defined medium conditioned over chick embryo heart cells promotes neurite outgrowth and rounding of the cell body (which becomes phase bright). A sensitive bioassay, based on counting the number of phase-bright neurons with processes at least equal to one cell body diameter after 20 hours in culture, was used to titrate neurite-promoting activity in heart conditioned medium. This activity is completely destroyed by exposure to trypsin. As little as 2 micrograms/ml total conditioned medium protein elicits a half-maximal response in this bioassay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Neurite outgrowth from cerebral cortical neurons is promoted by medium conditioned over heart cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't