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rdf:type |
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lifeskim:mentions |
umls-concept:C0010853,
umls-concept:C0016030,
umls-concept:C0023688,
umls-concept:C0031945,
umls-concept:C0041455,
umls-concept:C0086045,
umls-concept:C1167622,
umls-concept:C1280500,
umls-concept:C1514623,
umls-concept:C1516451,
umls-concept:C1749467,
umls-concept:C1879547
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pubmed:issue |
3
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pubmed:dateCreated |
1983-3-11
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pubmed:abstractText |
Efficient binding of native, soluble 125I-labeled type I rat collagen to mouse 3T3 fibroblast monolayers requires prior warming of the ligand to 35-37 degrees C for 10-30 min. Decreased binding at high ligand concentrations is ascribed to ligand-ligand interactions rather than to negative cooperativity. Addition of bacterial collagenase to monolayers labeled with the 125I-ligand releases a constant fraction (80%) of the bound ligand over a 2-h interval at 37 degrees C, indicating that little of the ligand becomes inaccessible by pinocytosis. Colchicine (10(-7) M) and vinblastine (5 X 10(-8) M) do not inhibit binding by morphologically intact monolayers. Cytochalasins and concanavalin A show dose-related inhibition of binding by intact monolayers that is due to a reduction in the number of available binding sites rather than to a change in binding site affinity. The collagen binding site on the fibroblast surface is proposed as an organizing center for the assembly of periodic type I collagen fibrils.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-1070005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-1092699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-13563501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-13845809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-165185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-185222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-205274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-206541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-218094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-28330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-4514314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-4551979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-6893482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-7015914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-711857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-7153246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-7189755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-7189756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-7195313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-7262331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-7448864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-769162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7153245-944583
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
747-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7153245-Animals,
pubmed-meshheading:7153245-Binding Sites,
pubmed-meshheading:7153245-Cell Line,
pubmed-meshheading:7153245-Cell Membrane,
pubmed-meshheading:7153245-Colchicine,
pubmed-meshheading:7153245-Collagen,
pubmed-meshheading:7153245-Concanavalin A,
pubmed-meshheading:7153245-Cytochalasins,
pubmed-meshheading:7153245-Fibroblasts,
pubmed-meshheading:7153245-Hot Temperature,
pubmed-meshheading:7153245-Mice,
pubmed-meshheading:7153245-Pinocytosis,
pubmed-meshheading:7153245-Protein Conformation,
pubmed-meshheading:7153245-Vinblastine
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pubmed:year |
1982
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pubmed:articleTitle |
Binding of soluble type I collagen to fibroblasts: effects of thermal activation of ligand, ligand concentration, pinocytosis, and cytoskeletal modifiers.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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