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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1983-3-24
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00093,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00094,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00096,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00158,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00159,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00160,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00161,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00162,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00163,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00164,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00165,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00166,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00167,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00168,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00169,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00170,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00171,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00172,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00173,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00174,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00175,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00177,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00178,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J00179,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K01239,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K01890,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K02544,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M18047,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M19067,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X00423
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pubmed:abstractText |
We have studied the structure and transcription of a cloned human beta-globin gene from a fetus diagnosed for beta 0 thalassemia. The sequence of the beta 0 gene differs from that of a normal beta-globin gene at positions 1 and 74 of the second intervening sequence (IVS2). The position 1 change alters the GT dinucleotide conserved at 5' splice sites, while the position 74 change is a common sequence polymorphism. When the cloned beta 0 gene is introduced into HeLa cells by use of an SV40-derived plasmid vector, two abnormally spliced cytoplasmic beta-globin RNAs are detected. The predominant RNA differs from normal beta-globin mRNA by the insertion of the first 47 nucleotides of IVS2 between exons 2 and 3. The less abundant RNA comprises the normal first exon spliced directly to the third. Analysis of nuclear RNA suggests that the beta 0 transcript is inefficiently spliced and that the removal of the two intervening sequences is coupled.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
903-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7151176-Base Sequence,
pubmed-meshheading:7151176-Cell Nucleus,
pubmed-meshheading:7151176-Cloning, Molecular,
pubmed-meshheading:7151176-Genes,
pubmed-meshheading:7151176-Globins,
pubmed-meshheading:7151176-HeLa Cells,
pubmed-meshheading:7151176-Humans,
pubmed-meshheading:7151176-RNA, Messenger,
pubmed-meshheading:7151176-RNA Processing, Post-Transcriptional,
pubmed-meshheading:7151176-RNA Splicing,
pubmed-meshheading:7151176-Thalassemia,
pubmed-meshheading:7151176-Transcription, Genetic
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pubmed:year |
1982
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pubmed:articleTitle |
A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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