Linked Life Data

7146751

Source:http://linkedlifedata.com/resource/pubmed/id/7146751

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1983-1-19
pubmed:abstractText
A method of red cell preservation by freezing at -25 degrees C is described. Glycerol is added to the red blood cells in the primary polyvinyl chloride plastic collection bag to achieve a concentration of 28 pr cent (W/V). The blood cells are concentrated by centrifugation and the supernatant glycerol is discarded. Glycerolized red cells are frozen and stored at -25 degrees C for 1 to 6 months. After thawing, Sodium chloride solutions are used to wash the red cells in the IBM Blood Processor 2991. The following parameters have been investigated before freezing, after thawing and washing and after storage of red blood cells at 4 degrees C for 24 hours: --Hemoglobin level --leukocytes and platelets --amount of 2-3 DPG and ATP. Preliminary data show that the in vitro quality of erythrocytes stored at -25 degrees C is well preserved for 4 months and that this simple method can be applied to blood preservation in any Blood Center.
pubmed:language
fre
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0338-4535
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
427-38
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
[Red cell preservation by freezing at -25 degrees C].
pubmed:publicationType
Journal Article, English Abstract