Linked Life Data

7144444

Source:http://linkedlifedata.com/resource/pubmed/id/7144444

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1983-1-19
pubmed:abstractText
The high potency with which acetylcholine (ACh) inhibits the binding of the specific muscarinic agonist, [3H]cis methyldioxolane ([3H]CD), has provided the basis for the development of a radioreceptor assay for estimation of ACh. A synaptosomal preparation of the rat cerebral cortex was used as a source of muscarinic receptors. When binding assays were run at 0 degree C, the IC50 value of ACh was approximately 5 x 10(-9)M, which corresponds to 2.5 - 10 pmoles of ACh, depending upon the assay volume. The ACh content of the rat cerebral cortex and corpus striatum was measured following fast microwave irradiation. By measuring the displacement of [3H]CD binding caused by aliquots of the supernatant from tissue homogenates and comparing the displacement values with an ACh standard curve, the ACh content of the cerebral cortex and corpus striatum was calculated to be 19 and 55 nmoles/g wet tissue weight, respectively.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0024-3205
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
347-54
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
A simple and rapid radio-receptor assay for the estimation of acetylcholine.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.