Linked Life Data

7139955

Source:http://linkedlifedata.com/resource/pubmed/id/7139955

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-1-19
pubmed:abstractText
Described is a rapid semi-automated technique for the quantitative extraction of bile acids and their conjugates from serum samples using commercially available reverse-phase octadecylsilane bonded silica cartridges. Serum is diluted with 4 volumes of 0.1 mol/l sodium hydroxide and the sample heated to 64 degrees C before application to the cartridge. Bile acids and their conjugates are adsorbed to the octadecylsilane bonded silica particles and reproducibly recovered by elution with a small volume of methanol. The technique is superior to extraction procedures using either Amberlite XAD resins or liquid-liquid partitioning, and gives a quantitative recovery of polar bile acid sulphates. The method is convenient and rapid and by using a specially designed apparatus it is possible to extract up to 60 samples/hour.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0009-8981
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
135-44
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
A rapid method for the quantitative extraction of bile acids and their conjugates from serum using commercially available reverse-phase octadecylsilane bonded silica cartridges.
pubmed:publicationType
Journal Article, Comparative Study