Linked Life Data

7138829

Source:http://linkedlifedata.com/resource/pubmed/id/7138829

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1983-1-7
pubmed:abstractText
PC12 is a clonal line of rat pheochromocytoma. The cells secrete dopamine and acetylcholine by a Ca2+-dependent process. The requirement for adenosine 5'-triphosphate (ATP) in secretion from PC12 was investigated. Cellular levels of ATP were decreased by brief treatments with inhibitors of glycolysis or oxidative phosphorylation. In order to deplete ATP stores below 40% of control values during a 5-min treatment with these compounds, it was necessary to inhibit both glycolysis and oxidative phosphorylation. Depletion of ATP pools to as little as 10% of control values did not result in any inhibition of dopamine or acetylcholine release evoked by 55 mM K+ or by carbachol. Rather, in many cases spontaneous and evoked releases were significantly increased under these conditions. The release observed in ATP-depleted cells was Ca2+ dependent. The data indicate that, unlike the situation with most other secretory cells, secretion from PC12 is independent of ATP. In PC12 some proteins involved in the secretion process may be altered so as to make the process independent of ATP; this may be due to the fact that PC12 is a tumor cell line. Phosphotyrosine-containing proteins, which are present in some other tumor cells, were not found in PC12.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4795-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Adenosine 5'-triphosphate independent secretion from PC12 pheochromocytoma cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.