Linked Life Data

7132058

Source:http://linkedlifedata.com/resource/pubmed/id/7132058

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-12-18
pubmed:abstractText
To study the role of the fixed anionic sites of the glomerular capillary wall in protein filtration, the negative charges were neutralized in vivo. With systemic infusion of the polycation protamine sulfate, glomerular staining for polyanion was reduced and protein excretion increased by 154%. To avoid systemic side effects in subsequent studies, small doses of a polycation were infused directly into one renal artery. The contralateral kidney was infused with the vehicle solution. Albumin excretion from the experimental kidneys in the first 1-hr collection after infusing 0.5 mg protamine sulfate was 24.3 +/- 6.3 micrograms/min/kidney (N = 13; P less than 0.01). Albuminuria declined during the subsequent 3 hr with a second infusion inducing a second proteinuric response. The degree and longevity of the albuminuric response was correlated directly to the dose of protamine sulfate. The polycations hexadimethrine and poly-l-lysine also induced proteinuria. The increased protein excretion consisted of albumin; the excretion of nonalbumin protein was identical in the experimental and control kidneys. Hemodynamic factors did not explain the increase in proteinuria. Morphologically, the polycation-treated kidneys showed scanty foot process fusion and a decrease in free negative sites in the lamina rarae of the glomerular basement membrane. The results strongly support an important role for glomerular charge in preventing filtration of circulating plasma albumin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0085-2538
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
127-35
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Glomerular charge and urinary protein excretion: effects of systemic and intrarenal polycation infusion in the rat.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't