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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1982-12-18
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pubmed:abstractText |
Muscarinic cholinergic receptors have been implicated in the regulation of bronchomotor tone, bronchial mucus secretion, and lung mast-cell mediator release. Rat lung membranes prepared by homogenization, sonication, and sedimentation of 37,000 g in sucrose have been demonstrated to possess muscarinic cholinergic receptors as assessed by radioligand binding employing l-quinuclidinyl[phenyl-4-3H]benzilate ([3H]QNB). QNB binding was saturable, stereoselective, and reversible in the presence of 1 microM atropine. The rat lung muscarinic receptor demonstrated a Kd of 0.268 +/- 0.126 (+/- SD) nM and a density of 35.4 +/- 4.6 fmol/mg protein by Scatchard analysis. The Hill coefficient was 1.015 +/- 0.085, indicating noncooperative binding. Muscarinic cholinergic receptors in the rat lung were equal in number to alpha 1-adrenergic receptors, but beta-adrenergic receptors are 14 times more prevalent as defined by [3H]prazosin and l-[propyl-2, 3-3H]dihydroalprenolol ([3H]DNA) binding. Dexamethasone (140 micrograms) administration in vivo caused an increase in all three receptor types by 24-48 h, suggesting steroids regulate not only the number of beta-receptors but also that of alpha- and cholinergic receptors. These data suggest new avenues by which steroids may regulate lung function.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenal Cortex Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Quinuclidinyl Benzilate,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cholinergic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0161-7567
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
731-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7129997-Adrenal Cortex Hormones,
pubmed-meshheading:7129997-Animals,
pubmed-meshheading:7129997-Binding, Competitive,
pubmed-meshheading:7129997-Dexamethasone,
pubmed-meshheading:7129997-Dogs,
pubmed-meshheading:7129997-Guinea Pigs,
pubmed-meshheading:7129997-Lung,
pubmed-meshheading:7129997-Male,
pubmed-meshheading:7129997-Membranes,
pubmed-meshheading:7129997-Quinuclidinyl Benzilate,
pubmed-meshheading:7129997-Rats,
pubmed-meshheading:7129997-Rats, Inbred Strains,
pubmed-meshheading:7129997-Receptors, Adrenergic,
pubmed-meshheading:7129997-Receptors, Cholinergic,
pubmed-meshheading:7129997-Receptors, Muscarinic
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pubmed:year |
1982
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pubmed:articleTitle |
Rat lung cholinergic receptor: characterization and regulation by corticosteroids.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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