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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1982-12-18
pubmed:abstractText
A new glycolipid was isolated from defatted cells of Pseudomonas paucimobilis IAM 12576, and called 'bound lipid'. The 'bound lipid' could not be extracted by hot phenol extraction, but could be extracted with hot chloroform/methanol after hydrolysis with 5% trichloroacetic acid. The 'bound lipid' was purified by thin-layer chromatography on silica gel plates using the solvent mixture CHCl3/CH3OH/CH3COOOH/ H2O (25:15:4:2, v/v). It consisted of glucosamine, 2-hydroxy myristic acid, galactose, mannose and uronic acid with ratios of 1.0:0.75:0.77:0.44:1.5, respectively, and other fatty acids besides 2-hydroxy myristic acid were not detected in the 'bound lipid'. 2-Hydroxy myristic acid was probably bound to glucosamine residues by amide linkage, because mild alkali treatment did not liberate the fatty acid. From these results, we discussed the possibility that the 'bound lipid' was some kind of lipid A of this bacteria.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
712
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
571-5
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Isolation of an unusual 'lipid A' type glycolipid from Pseudomonas paucimobilis.
pubmed:publicationType
Journal Article