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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1979-1-15
|
| pubmed:abstractText |
A method is described which permits the quantitative microanalysis of glycosphingolipids in the picomole range by HPLC of their perbenzoylated derivatives with detection at 230 nm. A linear gradient of 1 to 20% dioxane in hexane is used to elute from a Zipax column derivatives that contain up to four sugar residues. Residual absorption due to dioxane is negated by directing the solvent gradient through a pre-column, pre-injector high-pressure reference cell. This path generates a horizontal baseline with a negative and a positive deflection at the beginning and end of the gradient, respectively. Neutral glycosphingolipids can be quantitated either with N-acetyl-psychosine as internal standard or by comparison of peak areas to those of external standards.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-2275
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
910-3
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1978
|
| pubmed:articleTitle |
Quantitative microanalysis of perbenzoylated neutral glycosphingolipids by high-performance liquid chromatography with detection at 230 nm.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|