pubmed-article:7118995 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:7118995 | lifeskim:mentions | umls-concept:C0085080 | lld:lifeskim |
pubmed-article:7118995 | lifeskim:mentions | umls-concept:C0015283 | lld:lifeskim |
pubmed-article:7118995 | lifeskim:mentions | umls-concept:C0456205 | lld:lifeskim |
pubmed-article:7118995 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:7118995 | pubmed:dateCreated | 1982-12-2 | lld:pubmed |
pubmed-article:7118995 | pubmed:abstractText | The extent of exocytosis of pinocytic vesicle contents was studied in suspension-cultured Chinese hamster ovary (CHO) cells using horseradish peroxidase (HRP) as a pinocytic content marker. HRP was shown to be internalized via fluid-phase pinocytosis in CHO cells. After an HRP pulse of 2.5-10 min a rapid decrease of 30-50% in cell-associated HRP activity was observed within 10-20 min at 37 degrees C. During this time the loss of cell-associated HRP was accompanied by an equivalent increase in extracellular HRP. After this rapid exocytosis of HRP, the remaining peroxidase activity decreased with a t1/2 of 6-8 h, the known lysosomal half-life of HRP. In pulse-chase experiments HRP was chased into a nonexocytic compartment. Based on cell fractionation and electron microscopic experiments, this nonexocytic compartment was identified as a lysosome and the compartment from which exocytosis occurs as a pinosome. The occurrence of pinocytic content exocytosis in cultured fibroblasts suggests that exocytosis of pinocytic vesicle contents is a general phenomenon. | lld:pubmed |
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pubmed-article:7118995 | pubmed:language | eng | lld:pubmed |
pubmed-article:7118995 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7118995 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:7118995 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:7118995 | pubmed:month | Jun | lld:pubmed |
pubmed-article:7118995 | pubmed:issn | 0021-9525 | lld:pubmed |
pubmed-article:7118995 | pubmed:author | pubmed-author:AdamsC JCJ | lld:pubmed |
pubmed-article:7118995 | pubmed:author | pubmed-author:StorrieBB | lld:pubmed |
pubmed-article:7118995 | pubmed:author | pubmed-author:MaureyK MKM | lld:pubmed |
pubmed-article:7118995 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:7118995 | pubmed:volume | 93 | lld:pubmed |
pubmed-article:7118995 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:7118995 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:7118995 | pubmed:pagination | 632-7 | lld:pubmed |
pubmed-article:7118995 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:7118995 | pubmed:year | 1982 | lld:pubmed |
pubmed-article:7118995 | pubmed:articleTitle | Exocytosis of pinocytic contents by Chinese hamster ovary cells. | lld:pubmed |
pubmed-article:7118995 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:7118995 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:7118995 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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