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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1982-12-2
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pubmed:abstractText |
The extent of exocytosis of pinocytic vesicle contents was studied in suspension-cultured Chinese hamster ovary (CHO) cells using horseradish peroxidase (HRP) as a pinocytic content marker. HRP was shown to be internalized via fluid-phase pinocytosis in CHO cells. After an HRP pulse of 2.5-10 min a rapid decrease of 30-50% in cell-associated HRP activity was observed within 10-20 min at 37 degrees C. During this time the loss of cell-associated HRP was accompanied by an equivalent increase in extracellular HRP. After this rapid exocytosis of HRP, the remaining peroxidase activity decreased with a t1/2 of 6-8 h, the known lysosomal half-life of HRP. In pulse-chase experiments HRP was chased into a nonexocytic compartment. Based on cell fractionation and electron microscopic experiments, this nonexocytic compartment was identified as a lysosome and the compartment from which exocytosis occurs as a pinosome. The occurrence of pinocytic content exocytosis in cultured fibroblasts suggests that exocytosis of pinocytic vesicle contents is a general phenomenon.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-1030706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-14350504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-195744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-266721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-26836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-274729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-287030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-332066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-375982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-4112540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-4140194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-4347251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-455458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-4726306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-489568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-5279808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-5322983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-5656395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-567653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-6157480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-6928656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-6965100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-7198643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-7252122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-7419580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-922886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7118995-975211
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
632-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7118995-Animals,
pubmed-meshheading:7118995-Carbohydrates,
pubmed-meshheading:7118995-Cell Fractionation,
pubmed-meshheading:7118995-Cells, Cultured,
pubmed-meshheading:7118995-Cricetinae,
pubmed-meshheading:7118995-Cricetulus,
pubmed-meshheading:7118995-Cytoplasmic Granules,
pubmed-meshheading:7118995-Exocytosis,
pubmed-meshheading:7118995-Female,
pubmed-meshheading:7118995-Fibroblasts,
pubmed-meshheading:7118995-Hexosaminidases,
pubmed-meshheading:7118995-Horseradish Peroxidase,
pubmed-meshheading:7118995-Kinetics,
pubmed-meshheading:7118995-Lysosomes,
pubmed-meshheading:7118995-Ovary,
pubmed-meshheading:7118995-Pinocytosis
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pubmed:year |
1982
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pubmed:articleTitle |
Exocytosis of pinocytic contents by Chinese hamster ovary cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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