Linked Life Data

7118914

Source:http://linkedlifedata.com/resource/pubmed/id/7118914

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1982-12-3
pubmed:abstractText
The relative translational efficiency of the two human alpha-globin mRNAs, alpha 1 and alpha 2, was measured in vitro in a rabbit reticulocyte lysate system. To differentiate the translational products of these two mRNAs which normally encode an identical alpha-globin protein product, we used reticulocyte mRNA from a recently described Chinese subject. In this subject, an electrophoretically distinct alpha-globin mutant is encoded at the alpha 2 locus and both the alpha 1- and alpha 2-globin genes are deleted from the homologous chromosome (--/alpha 125Pro alpha). As in normal controls, the concentration of alpha 2-globin mRNA exceeded alpha 1 by approximately 3-fold. However, alpha 1- and alpha 2-globin proteins were synthesized by this reticulocyte mRNA at equal rates. This data suggests that the equal expression of the two alpha-globin genes observed in human erythrocytes results from a balance between the 3-fold excess of alpha 2-globin mRNA and a 3-fold higher translational efficiency of alpha 1-globin mRNA. The disparate translational efficiencies of the two alpha-globin mRNAs may be determined by the divergent structure of their 3' noncoding regions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
257
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11852-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Different rates of mRNA translation balance the expression of the two human alpha-globin loci.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't