Linked Life Data

7117670

Source:http://linkedlifedata.com/resource/pubmed/id/7117670

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1982-12-2
pubmed:abstractText
1. Galactokinase was purified from Chinese hamster liver. The purification process consisted of an initial biphasic partition separation followed by column chromatography on DEAE-cellulose. DEAE-Sephadex, Sephacryl S-200 and hydroxyapatite. 2. The enzyme was stabilized during the purification procedure by the inclusion of 10% glycerol, 1 mM phosphate and 20 mM beta-mercaptoethanol in all the buffer solutions. 3. Chromatography on hydroxyapatite separated two forms of galactokinase, one of which was purified to homogeneity using gel electrophoresis. 4. The purified galactokinase has a mol. wt of approximately 60,000 as determined by SDS gel electrophoresis and molecular sieving column chromatography. 5. The pH optimum is 7.4 and the Km for galactose is 1.16 x 10(-4) M.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0020-711X
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
719-26
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Isolation, purification and partial characterization of galactokinase from Chinese hamster liver.
pubmed:publicationType
Journal Article