Linked Life Data

711752

Source:http://linkedlifedata.com/resource/pubmed/id/711752

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1979-1-24
pubmed:abstractText
A simple radioisotopic assay for malonyl-CoA is described. The method is based on the malonyl-CoA-dependent incorporation of labeled acetyl-CoA into palmitic acid catalyzed by fatty acid synthetase in the presence of NADPH. Its main advantages over the more conventional spectrophotometric procedure is that it is extremely sensitive and allows the simultaneous determination of picomole quantities of malonyl-CoA in multiple tissue extracts. It should prove particularly suitable for studies on the regulation of lipid metabolism in isolated hepatocytes where the quantity of tissue available for analysis is frequently very small. Application of the method to the measurement of malonyl-CoA in livers from fed, fasted, and diabetic rats yielded values that were consistent with the recently postulated role of malonyl-CoA in the regulation of hepatic ketone body production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
253
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8291-3
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Hepatic malonyl-CoA levels of fed, fasted and diabetic rats as measured using a simple radioisotopic assay.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.